pc12 cells  (MedChemExpress)

Journal: Journal of Personalized Medicine

Article Title: Protection Against Cellular Toxicity from Rotenone Treatment by the Neuroprotective, Novel Multifunctional Antiparkinsonian Drug D-512

doi: 10.3390/jpm16020115

Figure Lengend Snippet: Neuroprotective effect of D-512 in a rotenone model: MN9D cells ( A ) or PC12 cells ( B ) were pretreated with different doses of D-512 for 1 h, followed by co-treatment with 1 μM rotenone for 24 h. Values shown are means ± SDs of three independent experiments. One-way ANOVA analysis followed by Tukey’s multiple comparison post hoc tests was performed. For ( A ), F (7, 56) = 166.5, #### p < 0.0001 compared to control, and **** p < 0.0001, ** p < 0.01 compared to the rotenone. For ( B ), F (7, 56) = 18.67, #### p < 0.0001 compared to control and *** p < 0.001, * p < 0.05 compared to the rotenone.

Article Snippet: Rat adrenal pheochromocytoma PC12 cell lines (ATCC, Manassas, VA, USA, CRL1721.1TM) cells were purchased from ATCC.

Techniques: Comparison, Control

Journal: Journal of Personalized Medicine

Article Title: Protection Against Cellular Toxicity from Rotenone Treatment by the Neuroprotective, Novel Multifunctional Antiparkinsonian Drug D-512

doi: 10.3390/jpm16020115

Figure Lengend Snippet: Effect of D-512 in reversing rotenone-induced mitochondrial membrane stress: MN9D cells ( A ) and PC12 cells ( B ) were pretreated with different doses of D-512 for 1 h, followed by co-treatment with 1 μM rotenone for 24 h. Mitochondrial health was measured using JC1 staining, and the ratios of red/green were plotted. Values shown are means ± SDs of three independent experiments. One-way ANOVA analysis followed by Tukey’s multiple comparison post hoc test was performed. For ( A ), F (7, 16) = 7.78, ** p < 0.01, * p < 0.05 compared to the rotenone, and for ( B ), F (7, 56) = 62.89, **** p < 0.0001 compared to the rotenone.

Article Snippet: Rat adrenal pheochromocytoma PC12 cell lines (ATCC, Manassas, VA, USA, CRL1721.1TM) cells were purchased from ATCC.

Techniques: Membrane, Staining, Comparison

Journal: Journal of Personalized Medicine

Article Title: Protection Against Cellular Toxicity from Rotenone Treatment by the Neuroprotective, Novel Multifunctional Antiparkinsonian Drug D-512

doi: 10.3390/jpm16020115

Figure Lengend Snippet: Effect of D-512 pretreatment on rotenone-induced ROS release: PC12 cells were treated with 1 µM rotenone, D-512 (5 µM and 10 µM), or D-512 (5 µM and 10 µM) with 1 µM rotenone. ROS was measured by DCFDA. Values shown are means ± SDs of three independent experiments. One-way ANOVA analysis followed by Tukey’s multiple comparison post hoc test was performed, F (5, 42) = 75.44, **** p < 0.0001, *** p < 0.001 compared to the 1 µM rotenone; #### p < 0.0001 compared to control.

Article Snippet: Rat adrenal pheochromocytoma PC12 cell lines (ATCC, Manassas, VA, USA, CRL1721.1TM) cells were purchased from ATCC.

Techniques: Comparison, Control

Journal: Journal of Personalized Medicine

Article Title: Protection Against Cellular Toxicity from Rotenone Treatment by the Neuroprotective, Novel Multifunctional Antiparkinsonian Drug D-512

doi: 10.3390/jpm16020115

Figure Lengend Snippet: Western blot studies on the effect of D-512 in decreasing ERK activity induced by rotenone toxicity: PC12 cells were treated with 1µM rotenone for different time points (30 min, 1 h, 2 h, 4 h, 6 h) ( A , C ). Dose-dependent effect of pretreatment with D-512, followed by co-treatment with rotenone on ERK levels in PC12 cells. PC12 cells were pretreated with different doses of D-512 (10 µM and 5 µM) for 24 h, followed by co-treatment with 1 μM rotenone for 30 min. The membranes were probed with p-ERK and t-ERK antibodies ( B , D ). Actin was used as a loading control. Values shown are means ± SDs of three independent experiments. One-way ANOVA analysis followed by Tukey’s multiple comparison post hoc test was performed. For ( C ) F (5, 12) = 6.93, ** p < 0.01 compared to the control, and for ( D ) F (4, 10) = 11.41, ** p < 0.01 compared to the rotenone.

Article Snippet: Rat adrenal pheochromocytoma PC12 cell lines (ATCC, Manassas, VA, USA, CRL1721.1TM) cells were purchased from ATCC.

Techniques: Western Blot, Activity Assay, Control, Comparison